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. 2019 Jan 4;9:3050. doi: 10.3389/fimmu.2018.03050

Figure 2.

Figure 2

Melanoma cell lysates induce activation of XBP1s and XBP1s-dependent genes, but not RIDD. (A) FL-DCs were left untreated (NT) or were stimulated with 100 μg/ml MEL for 8 h. Expression of Erp44, Sec61a, Bloc1s1, and Tapbp mRNA was measured by qPCR relative to L27 expression, and depicted as fold of induction to the NT condition. Data in graphs depicts three to five independent experiments. (B) Expression of VenusFP in FL-DCs generated from ERAI mice and stimulated with AIM-V medium (control medium) 100 ug/ml MEL, 100 ug/ml human ovarian cancer lysate (OvCa) and 100 ug/ml human gallbladder cancer cells lines (GBCa) for 24 h. Data in graphs depicts the MFI of cDC1 FL-DCs (XCR1+) and each symbol in the graph represents data derived from one independent experiment. For all error bars represent mean ± SEM. *p < 0.05, ***p < 0.001 (paired Student's t-test).