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. 2019 Jan 4;9:3050. doi: 10.3389/fimmu.2018.03050

Figure 6.

Figure 6

IRE1α endonuclease activity in GMCSF-BMDCs promotes cross-presentation of tumor-associated antigens in vivo. (A,B) Western Blot analysis of IRE1 levels in FL-DCs and GMCSF BMDCs of IRE1trunc or ctrl DC mice. (C) Phenotype of GMCSF BMDCs from IRE1trunc or ctrl DC mice at day 8 of culture (gate on CD11c+ cells). (D) In vivo proliferation of OT-I CD8 T cells (CD45.1+). 2 × 106 CD8 T cells stained with CellTrace Violet (CTV) were adoptively transferred into congenic mice. One day later mice were injected i.v. with 2.5 × 105 GMCSF BMDCs, from IRE1trunc or ctrl DC mice pulsed with 100 μg/ml B16 lysate plus 200 μg/ml OVA. Histograms represent the proliferation of transferred cells (CD8+ CD45.1+) in the spleen. (E) The graph represents the percentage of proliferation of CellTrace Violet-labeled cells. Each symbol in the graph represents data from an individual mouse. Error bars represent mean ± S.E.M. *p < 0.05 determined by Mann–Whitney test.