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. 2019 Jan 10;12:518. doi: 10.3389/fncel.2018.00518

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Copyright © 2019 Grassi, Santoro, Umbach, Grosso, Oliviero, Neri, Conti, Ala, Provero, DiCunto and Merlo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of depletion of Elavl3 in differentiating ANS cells. (A) RealTime qPCR analysis to detect the abundance of Elavl3 mRNA in ANS cells treated with the control (black) or anti-Elavl3 (white) siRNA. (B) Western blot analysis of ANS cells treated with the control siRNA or with the anti-Elavl3 siRNA, to confirm the downmodulation of Elavl3 protein (top). Staining with anti-actin on the same blot is used as loading control (bottom). (C) Real Time qPCR analysis to detect the relative abundance of the longer vs. the common variants of Pes1, Hnrnpa0, Gng2 and Tex2 mRNAs, comparing siRNA-treated vs. control-treated ANS cells, maintained in differentiation conditions. (D) Real-Time qPCR analysis to detect the mRNA abundance of the differentiation genes GAD1, Tubβ3, NeuN and Map2 in ANS cells treated with the control (black bars) or anti-Elavl3 (open bars) siRNA. *p < 0.05.