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. 2019 Jan 11;9:3140. doi: 10.3389/fimmu.2018.03140

Figure 4.

Figure 4

PTEN is a potential target of miR-19b. (A) Cell signaling pathways involving miR-19b target genes were assessed through a bioinformatics analysis (http://diana.imis.athena-innovation.gr/). The FOXO signaling pathway (Red) is a major cellular signaling pathway playing a key role in cell cycle regulation. (B) The expression of miR-19b was enhanced by mimics in Jurkat cells. Four genes in the FOXO pathway were detected through qRT-PCR. (C–F) The expression of PTEN was inhibited by introducing 20 μM PTEN siRNA to isolated CD8+T cells. (C) After transfection (24 h), CD8+T cells were labeled with Cell Trace™ Violet and stimulated using anti-CD3/CD28 (3 μg/ml) for 5 days. The proliferation of CD8+T cells was determined. (D) After transfection (24 h), CD8+ T cells were stimulated using anti-CD3/CD28 (3 μg/ml) for 48 h and the rate of apoptosis of CD8+ T cells was determined. (E,F) After transfection (24 h), CD8+ T cells were stimulated using anti-CD3/CD28 (3 μg/ml) for 24 h and the intracellular expression of IFN-γ (E) and granzyme B (F) was determined. n = 6 for each group in (C), n = 7 for each group in (D), n = 6 for each group in (E,F). *P < 0.05. **P < 0.01. ***P < 0.001, respectively.