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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Circ Cardiovasc Imaging. 2019 Jan;12(1):e007835. doi: 10.1161/CIRCIMAGING.118.007835

Figure 1. Affinity study of calcium salts with two markers for microcalcification - a near infrared fluorescence (NIRF) calcium tracer, and 18F-fluoride.

Figure 1.

A, Signal quantification of NIRF tracer for individual calcium salts, and a Kruskal-Wallis test with Dunn’s multiple comparison test: Control – no mineral added (0.0±0.0, 0.0% of HA), HA – Hydroxyapatite (0.422±0.304, p<0.0001), PO4 – Calcium Phosphate (0.008±0.011, 1.8% of HA, p=0.1625), PP – Calcium Pyrophosphate (0.062±0.066, 14.8% of HA, p<0.001), Ox – Calcium Oxalate (0.006±0.008, 1.5% of HA, p=0.0133), CO3 – Calcium Carbonate (0.006±0.008, 1.5% of HA, p=0.1237). B, Signal quantification of radioactively labelled 18F-fluoride for different calcium salts, and a Kruskal-Wallis test with Dunn’s multiple comparison test: Control (0.007×105±0.010×105 CPM, 0.05% of HA), HA (13.9×105±0.7×105 CPM, p=0.0066), PO4 (0.27×105±0.08×105 CPM, 1.9% of HA, p=0.3323), PP (8.91×105±0.94×105 CPM, 64.0% of HA, p=0.0581), Ox (0.13×105±0.01×105 CPM, 0.9% of HA, p>0.9999), CO3 (0.006×105±0.005×105 CPM, 0.04% of HA, p>0.9999). C, Fluorescence images of different calcium salts after incubation with NIRF calcium tracer (scale bars, 100 µm). D, Autoradiograms of different calcium salts after incubation with 18F-fluoride (scale bars, 0.25 mm).