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. 2019 Jan 14;13:317–326. doi: 10.2147/DDDT.S185514

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© 2019 Tong et al. This work is published and licensed by Dove Medical Press Limited

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YB-1 mediates glioma cells response to TMZ.

Notes: (A) The vector or YB-1-overexpressed U87 (p53wt) and DK-MG (p53wt) cells were treated with various doses (0, 50, 100, 200 µM) of TMZ for 48 hours, and cell viability was determined by CCK-8. *P<0.05 vs the control group. (B) U87 and DK-MG cells were transfected with siControl or siYB-1, followed by treatment with various doses (0, 50, 100, 200 µM) of TMZ for 48 hours, and cell viability was determined by CCK-8. The protein (C) and mRNA (D) levels of YB-1, MDM2, and p53 were analyzed by Western blot and qRT-PCR after overexpression or knockdown of YB-1 in U87 and DK-MG cells. *P<0.05, **P<0.01, ***P<0.001 vs the control group.

Abbreviations: CCK-8, cell counting kit-8; qRT-PCR, quantitative real-time PCR; TMZ, temozolomide.

YB-1 mediates glioma cells response to TMZ.

Notes: (A) The vector or YB-1-overexpressed U87 (p53wt) and DK-MG (p53wt) cells were treated with various doses (0, 50, 100, 200 µM) of TMZ for 48 hours, and cell viability was determined by CCK-8. *P<0.05 vs the control group. (B) U87 and DK-MG cells were transfected with siControl or siYB-1, followed by treatment with various doses (0, 50, 100, 200 µM) of TMZ for 48 hours, and cell viability was determined by CCK-8. The protein (C) and mRNA (D) levels of YB-1, MDM2, and p53 were analyzed by Western blot and qRT-PCR after overexpression or knockdown of YB-1 in U87 and DK-MG cells. *P<0.05, **P<0.01, ***P<0.001 vs the control group.

Abbreviations: CCK-8, cell counting kit-8; qRT-PCR, quantitative real-time PCR; TMZ, temozolomide.