Table 1.

Primers used for the analysis of the expression of NCR-related genes in H. vineae.

Gene	Name	Oligonucleotide sequence (5′–3′ end)
ACT1	ACT-F	GGCTTCTTTGACCACTTTCCAA
	ACT-R	GATGGACCACTTTCGTCGTATTC
AGP1	AGP1-F	ATTGCTGGGTGACGGTTCTT
	AGP1-R	TGACATTGGTAGCGGCAATAAC
GAP1	GAP1-F	CAAAGGTTTGCCATCTGTCATC
	GAP1-R	TGCAGAGTTACCGACAGACAACA
MEP2	MEP2-F	TCGATGACGGGTTGGATGTT
	MEP2-R	CATAACGGCACCACTTAAACCA
PUT2	PUT2-F	GATACGACATGTTGGCAGCAA
	PUT2-R	TTTCGCCTTGGAAAACGTTT

The primer design for the H. vineae genes was performed using Primer Express software (Primer Express 3.0 Applied Biosystems). The primers used for S. cerevisiae have been previously described by Beltran et al. (2004) and Gutiérrez et al. (2013).