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. 2018 Dec 20;3(24):e97293. doi: 10.1172/jci.insight.97293

Figure 10. LPA4 activation decreases PPARγ-related gene expression in C3H10T1/2- and mouse embryonic fibroblast–derived (MEF-derived) adipocytes.

Figure 10

(A and B) Effects of octadecenyl phosphate (ODP) on expression of adipogenesis marker genes (A) and genes regulated by phosphorylation of PPARγ (B) in C3H10T1/2-derived adipocytes. C3H10T1/2 cells were differentiated into adipocytes, serum-starved for 24 hours, and treated with 2.5 μM ODP for 24 hours (n = 4). (C and D) Effects of ODP on expression of adipogenesis marker genes in WT-mouse MEF-derived adipocytes (C) and Lpar4-KO MEF-derived adipocytes (D). MEFs were differentiated into adipocytes, serum-starved for 24 hours, and treated with 10 μM ODP for 24 hours (n = 4). Data shown are representative of 2 independent experiments. Data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.001, unpaired Student’s t test.