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AG11395 cells were transfected with different WRN mutant or fragmented WRN or full length WRN. Cells were then treated with tunicamycin for 8 h along with complete medium. (A) Immunobloting of total cellular extract for autophagy marker proteins. (B) Graphical representation of ratio LC3BII and LC3BI of all conditions. 3 western blots from 3 separate sets of samples were used.
