Table 2.
Compounds | Conc. (ng/mL) | Intra-day (n = 6) |
Inter-day (n = 18) |
||||
---|---|---|---|---|---|---|---|
Measured conc. (ng/mL) | RE% | RSD% | Measured conc. (ng/mL) | RE% | RSD% | ||
Sweroside | 50 | 50.9 ± 5.1 | 5.1 | 10.0 | 51.3 ± 1.0 | 2.7 | 2.0 |
200 | 203.1 ± 25.1 | 3.8 | 12.3 | 204.1 ± 3.1 | 2.1 | 1.5 | |
1600 | 1617.3 ± 143.0 | −10.1 | 8.8 | 1524.2 ± 89.5 | −4.7 | 5.9 | |
Magnoflorine | 3 | 3.0 ± 0.3 | 6.8 | 8.3 | 3.1 ± 0.1 | 4.6 | 2.9 |
150 | 155.1 ± 10.9 | 8.1 | 7.0 | 159.2 ± 3.7 | 6.2 | 2.3 | |
1200 | 1090.8 ± 35.8 | −11.0 | 3.3 | 1077.7 ± 11.6 | −10.2 | 1.1 | |
Epimedin C | 5 | 5.1 ± 0.5 | −2.6 | 9.1 | 5.0 ± 0.1 | 0.7 | 2.9 |
200 | 212.1 ± 13.5 | 10.8 | 6.4 | 217.0 ± 4.7 | 8.5 | 2.2 | |
1600 | 1486.2 ± 55.4 | −11.6 | 3.7 | 1451.7 ± 36.0 | −9.3 | 2.5 | |
Akebia saponin D | 50 | 50.3 ± 5.2 | 8.4 | 10.3 | 52.6 ± 2.0 | 5.2 | 3.9 |
200 | 215.2 ± 19.2 | 11.0 | 8.9 | 216.0 ± 5.6 | 8.0 | 2.6 | |
1600 | 1460.9 ± 71.5 | −11.0 | 4.9 | 1427.9 ± 31.7 | −10.8 | 2.2 | |
Psoralen | 10 | 10.1 ± 1.0 | −6.0 | 10.0 | 9.9 ± 0.4 | −1.4 | 4.0 |
300 | 289.4 ± 13.5 | 5.2 | 4.6 | 304.4 ± 13.5 | 1.5 | 4.4 | |
2400 | 2507.9 ± 185.9 | −10.7 | 7.4 | 2343.1 ± 184.5 | −2.4 | 7.9 | |
Isopsoralen | 10 | 10.5 ± 1.1 | 4.3 | 10.4 | 10.0 ± 0.4 | −0.2 | 4.1 |
150 | 145.4 ± 12.5 | −3.1 | 8.6 | 150.2 ± 4.2 | 0.1 | 2.8 | |
1200 | 1172.3 ± 75.2 | −9.3 | 6.4 | 1162.5 ± 69.5 | −3.1 | 6.0 | |
Neobavaisoflavone | 1 | 1.0 ± 0.1 | 2.4 | 8.9 | 1.0 ± 0.1 | 1.6 | 1.9 |
30 | 31.4 ± 2.4 | 9.5 | 7.6 | 32.1 ± 0.7 | 7.0 | 2.3 | |
240 | 230.3 ± 10.0 | −11.9 | 4.3 | 220.7 ± 9.4 | −8.1 | 4.2 | |
Icariside II | 1 | 1.1 ± 0.1 | 8.0 | 4.8 | 1.1 ± 0.1 | 7.8 | 0.9 |
20 | 20.8 ± 2.0 | 8.3 | 9.5 | 21.3 ± 0.5 | 6.5 | 2.2 | |
160 | 146.5 ± 5.3 | −11.2 | 3.6 | 149.7 ± 9.1 | −10.2 | 6.1 | |
Corylin | 2.5 | 2.4 ± 0.3 | 5.6 | 11.4 | 2.5 ± 0.1 | 0.4 | 5.4 |
10 | 10.2 ± 1.1 | 3.2 | 10.7 | 10.2 ± 0.1 | 1.9 | 1.1 | |
80 | 76.0 ± 5.2 | −8.3 | 6.8 | 75.7 ± 2.1 | −5.4 | 2.8 | |
Psoralidin | 2 | 2.1 ± 0.2 | 4.7 | 10.0 | 2.1 ± 0.1 | 2.4 | 2.5 |
50 | 55.3 ± 2.5 | 10.6 | 4.5 | 54.7 ± 0.5 | 9.5 | 0.9 | |
400 | 360.4 ± 11.9 | −12.7 | 3.3 | 354.7 ± 5.6 | −11.3 | 1.6 | |
Bavachinin | 2 | 1.9 ± 0.2 | −9.5 | 9.1 | 1.9 ± 0.1 | −5.6 | 3.8 |
10 | 9.9 ± 1.0 | 8.7 | 10.2 | 10.4 ± 0.5 | 4.1 | 4.7 | |
80 | 73.0 ± 3.6 | −12.3 | 4.9 | 72.1 ± 1.7 | −9.8 | 2.4 |
Conc. means concentration.