Table 1. List of proteins identified in Naja annulifera venom by in-solution trypsin digestion and LC-MS/MS analysis*.
| Protein family | Protein ID** | Protein | # Peptides |
|---|---|---|---|
| Snake three-finger toxin family | P01454 | Cytotoxin 9 | 14 |
| P62394 | Cytotoxin 11 | 6 | |
| P01421 | Short neurotoxin 4 | 10 | |
| P68419 | Short neurotoxin 1 | 12 | |
| P01456 | Cytotoxin 1 | 13 | |
| Q9W717 | Neurotoxin-like protein NTL2 | 2 | |
| P01463 | Cytotoxin 2 | 7 | |
| P01453 | Cytotoxin 10 | 10 | |
| P29181 | Weak neurotoxin 7 | 2 | |
| P01401 | Weak toxin CM-11 | 8 | |
| P01399 | Weak toxin CM-13b | 4 | |
| P01462 | Cytotoxin 2 | 11 | |
| P81782 | Bucandin | 3 | |
| P01400 | Weak toxin S4C11 | 6 | |
| P25674 | Long neurotoxin 1 | 7 | |
| P25678 | Weak toxin CM-2a | 3 | |
| P01457 | Cytotoxin 5 | 6 | |
| Q9W6W6 | Cytotoxin 10 | 5 | |
| P01422 | Short neurotoxin 2 | 7 | |
| P82462 | Muscarinic toxin-like protein 1 | 2 | |
| R4FK68 | 3FTx-Pse-105 | 1 | |
| P01461 | Cytotoxin 4 | 7 | |
| R4G7H8 | 3FTx-Fur-10 | 1 | |
| P01464 | Cytotoxin 5 | 6 | |
| P01388 | Long neurotoxin 2 | 3 | |
| P01389 | Long neurotoxin 1 | 5 | |
| P01426 | Short neurotoxin 1 | 5 | |
| CRISP family | Q7T1K6 | Cysteine-rich venom protein natrin-1 | 6 |
| P84808 | Cysteine-rich venom protein kaouthin-2 | 5 | |
| P0DL15 | Cysteine-rich venom protein annuliferin-b (Fragment) | 5 | |
| P84807 | Cysteine-rich venom protein 25-A (Fragment) | 1 | |
| Q8JI38 | Cysteine-rich venom protein latisemin | 2 | |
| F2Q6F2 | Cysteine-rich seceretory protein Dr-CRPK | 2 | |
| C1JZW4 | Opharin | 2 | |
| Q7ZT98 | Cysteine-rich venom protein ophanin | 5 | |
| Phospholipase A2 family | B2BRS4 | Truncated putative phospholipase A2 | 1 |
| P25498 | Acidic phospholipase A2 E | 1 | |
| Q92085 | Neutral phospholipase A2 B | 1 | |
| P00605 | Phospholipase A2 basic | 2 | |
| P00600 | Acidic phospholipase A2 DE-II | 4 | |
| SVMP family | D6PXE8 | Zinc metalloproteinase-disintegrin-like atrase-B | 5 |
| D5LMJ3 | Zinc metalloproteinase-disintegrin-like atrase-A | 7 | |
| Q10749 | Snake venom metalloproteinase-disintegrin-like mocarhagin | 6 | |
| P82942 | Hemorrhagic metalloproteinase-disintegrin-like kaouthiagin | 4 | |
| D3TTC2 | Zinc metalloproteinase-disintegrin-like atragin | 6 | |
| Type-B carboxylesterase/lipase family | A0A098LYB5 | Carboxylic ester hydrolase | 5 |
| R4FKE6 | Carboxylic ester hydrolase (Fragment) | 4 | |
| Q92035 | Acetylcholinesterase | 3 | |
| A0A098LY86 | Carboxylic ester hydrolase (Fragment) | 2 | |
| Phosphodiesterase family | A0A2D0TC04 | Snake venom phosphodiesterase (PDE) | 14 |
| A0A0F7YYZ8 | Phosphodiesterase | 9 | |
| Lectin family | U3FVL3 | Vesicular integral-membrane protein VIP36 | 1 |
| R4G314 | LP-Pse-6 | 1 | |
| Flavin monoamine oxidase family | A0A2R4N4Q6 | Amine oxidase (Fragment) | 14 |
| R4FID0 | Amine oxidase | 8 | |
| NGF family | P61899 | Venom nerve growth factor | 7 |
| Q5YF89 | Venom nerve growth factor 2 | 4 | |
| Ohanin/vesprin family | P82885 | Thaicobrin | 6 |
| A0A182C6D0 | Ohanin | 4 | |
| 5'-nucleotidase family | U3FYP9 | Ecto-5-nucleotidase 1c | 13 |
| Ankyrin repeat | V8NWT6 | Ankyrin repeat domain-containing protein 34B (Fragment) | 1 |
| Carbohydrate/starch-binding module (family 21) | V8N6R7 | Protein phosphatase 1 regulatory subunit 3A (Fragment) | 1 |
| DEATH domain, DD | V8P0T5 | Tumor necrosis factor receptor superfamily member 11B | 3 |
| Endonuclease family | V8N4Y2 | Endonuclease domain-containing 1 protein | 5 |
| Glutathione peroxidase family | V8P395 | Glutathione peroxidase (Fragment) | 10 |
| Glycosyl hydrolase 56 family (hyaluronidase) | U3FYQ4 | Hyaluronidase | 3 |
| LDH/MDH superfamily | V8P6K5 | L-lactate dehydrogenase | 1 |
| Pyruvate kinase family | V8P2C8 | Pyruvate kinase | 1 |
| SVSP family | A8QL57 | Snake venom serine protease BmSP (Fragment) | 2 |
| Thiolase family | V8NMW3 | Non-specific lipid-transfer protein | 1 |
| Tudor domain | V8NEV9 | Tudor domain-containing protein 6 (Fragment) | 1 |
| Venom complement C3 homolog family | Q91132 | Cobra venom factor | 18 |
| Venom Kunitz-type family | P00986 | Kunitz-type serine protease inhibitor 2 | 5 |
| Unknown | A0A2D4GN98 | Uncharacterized protein (Fragment) | 1 |
| V8NUN2 | Transmembrane protein 14C | 1 | |
| A0A2D4JG71 | Uncharacterized protein (Fragment) | 1 | |
| A0A2D4KYJ8 | Uncharacterized protein (Fragment) | 1 | |
| A0A2D4H759 | Uncharacterized protein (Fragment) | 1 | |
| A0A2D4IS21 | Uncharacterized protein (Fragment) | 1 |
*Only proteins identified in at least two experimental replicates, with at least 1 unique peptide and posterior error probability ≤ 0.01 are listed.
**The first protein entry was selected as a representative of each Protein Group as identified using the MaxQuant software package.