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. 2019 Jan 18;14(1):e0207836. doi: 10.1371/journal.pone.0207836

Fig 4. BMN 250 exhibits CI-MPR-dependent and receptor-independent uptake in rat-derived neurons, astrocytes and microglia.

Fig 4

A, B and C: Representative uptake curves of BMN 250 in the absence (black circles) or presence (grey squares) of 4 uM IGF2, or rhNAGLU (black triangles, hashed line) as indicated, in duplicate cultures of normal rat-derived primary cultures of cortical neurons (A; N = 1 repeat) astrocytes (B; N = 3 repeats) and microglia (C; N = 3 repeats). BMN 250 Kuptake in rat cortical neurons = 5.0 nM, Vmax = 1,055 nmol/hr/mg; BMN 250 Kuptake in astrocytes = 3.4 nM, Vmax = 5,404 nmol/hr/mg; BMN 250 Kuptake in microglia = 2.6 nM, Vmax = 12,383 nmol/hr/mg; BMN 250 Kuptake in microglia in the presence of 4 uM IGF2 = 21.4 nM, Vmax = 5148 nmol/hr/mg; rhNAGLU Kuptake in microglia = 4.5 nM; Vmax = 5424 nmol/hr/mg. Data sets for Fig 4 are shown in the supporting information section.