Fig 2. Lapatinib works in conjunction with Th1 cytokines to increase percentage of non-viable cells.

Cultured SK-BR-3, BT-474 and MDA-MB-468 cells were treated either with Th1 cytokines (TNF-α plus IFN-γ; 20 and12.5ng/ml respectively), Lapatinib (200nM), Lapatinib plus Th1 cytokines or left untreated (control). After 72 hours, cells were harvested, stained with Trypan Blue dye and percentage of viable and non-viable cells enumerated microscopically with the aid of a hemocytometer. Shown are composite data from three independent experiments with each cell line expressed as the percentage stained (i.e. non-viable) cells of total cells counted. Statistical significance was determined by one-way ANOVA on percent dead followed by the Holm-Sidak multiple comparison test.