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. 2019 Jan 18;14(1):e0210209. doi: 10.1371/journal.pone.0210209

Fig 7. Lapatinib works in conjunction with Th1 cytokines to maximize indicators of apoptotic cell death in drug-resistant cell lines.

Fig 7

Cultured HCC1419, JIMT-1 and MDA-MB-453 cells were treated either with Th1 cytokines (TNF-α plus IFN-γ; 20 and 12.5ng/ml respectively), Lapatinib (2μM), Lapatinib plus Th1 cytokines or left untreated (control). After 72 hours incubationcells were harvested and stained with FITC-Annexin V and propidium iodide (PI) and subjected to FACS analysis. Two-color dot-plot analysis displays percentage of gated cells in each quadrant (double-staining cells represent late apoptotic events).