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. 2019 Jan 18;14(1):e0210665. doi: 10.1371/journal.pone.0210665

Fig 3. CD24+ Ptch1lox/lox;GFAPcre cells co-label with markers of neural progenitors and granule cell precursors.

Fig 3

(A-R) Co-immunolabelling of cytospun flow-sorted CD24+ and CD24-Ptch1lox/lox;GFAPcre primary cells with markers of progenitor and differentiated cells. Adjacent bar graphs illustrate the percentage of CD24+ (white bars) and CD24- (black bars) cells that co-labelled with their respective secondary marker. Co-labelled antibodies were PCNA: proliferating cells (A-C), Sox2: stem/progenitor cells (D-F), NeuN: mature neurons (G-I), βIIItubulin: neural progenitors (J-L), Pax6: radial glial progenitors (M-O) and BLBP: radial glial cells (P-R). Inset images show magnified images of the co-staining. Scale bar A, H, J, K and P 200μm, scale bar B, D, E, G, M, N and Q 100μm. NS: non-significant, *p<0.05, **p<0.01, ***p<0.001.