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. 2019 Jan 18;14(1):e0211156. doi: 10.1371/journal.pone.0211156

Fig 4. Sucrose gradient centrifugation of PDR8, PDR2 and WBC1 in P. pastoris.

Fig 4

P. pastoris crude membranes expressing WBC1, PDR8, PDR2 and MDR3 and S. cerevisiae crude membranes expressing PDR5 were separated via ultracentrifugation through a continuous sucrose gradient. Samples were analyzed by SDS-PAGE and immunoblotting (anti-His-tag antibody, anti-PDR5 antibody, C219 antibody). The analyzed proteins are co-localized with the plasma membrane proteins MDR3 and PDR5. The displayed results were assembled from different Western Blots. Entire blots can be found in the supplemental material (S1–S10 Figs).