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. Author manuscript; available in PMC: 2019 Jan 18.
Published in final edited form as: Adv Biosyst. 2017 Jan 31;1(1-2):1600019. doi: 10.1002/adbi.201600019

Figure 5. Mechanical force modulates Dll4 expression in HUVEC network in vitro.

Figure 5

(A-B) Western blot analysis of Notch1 and Dll4 expressions in HUVEC networks treated with Y-27632 and nocodazole. Data are representative from three independent experiments and are expressed as mean ± s.e.m. (n = 3; ns, not significant; *P<0.05, **P<0.01; unpaired Student’s t-test). (C) Notch1 and Dll4 mRNA expressions in HUVEC networks in vitro. Gene expressions were measured by a gold nanorod biosensor with locked nucleic acid probes targeting Notch1 and Dll4 mRNA. Data are representative from three independent experiments and each data point represents at least 150 cells (ns, not significant; *P<0.05, **P<0.01; unpaired Student’s t-test). (D) Western blot analysis of Notch1 and Dll4 expression in HUVECs pretreated with Notch 1 siRNA or Dll4 siRNA. Data are representative from three independent experiments. (E-F) Mean cord lengths of siRNA-treated HUVEC networks without (E, n = 10, 9 hours after cell seeding) or with (F, n = 12, 9 hours after cell seeding) Y-27632. Data are expressed as mean ± s.e.m. (ns, not significant; *P<0.05, **P<0.01, ***P<0.001; unpaired Student’s t-test).