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. 2019 Jan 18;15(1):e1007819. doi: 10.1371/journal.pgen.1007819

Fig 3. SALK and SAIL T-strand sequences show divergent epigenomic signatures.

Fig 3

Transgene activity was tested by exposing plants to the respective selective agent: (a) SALK_059379 grown on media containing the antibiotic kanamycin (50ug/uL) (or empty control) and (b) the SAIL_232 sprayed with the herbicide Finale™ at two different concentrations through the middle of the tray. All phenotypes are compared to the WT Col-0 (a,b). Analysis of expression and epigenetic signatures on the corresponding T-DNA sequence is captured in genome browser shots for SALK_059379 plasmid pROK2 (c) and SAIL_232 plasmid pCSA110 (d): Illumina read mapping of bisulfite sequencing, RNA-seq and different small RNA species. Quantification of individual siRNA read length against individual parts of the two plasmid sequences are reported in (e = pROK2) and (f = pCSA110). GUS staining of leaves and flowers of SAIL_232 (g), with Col-0 (h) and SALK_059379 (i) as control.