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. Author manuscript; available in PMC: 2020 Jan 17.
Published in final edited form as: Mol Cell. 2018 Dec 6;73(2):314–324.e4. doi: 10.1016/j.molcel.2018.11.002

Fig. 2. SKI3 deletion prevents the pat1Δ-mediated decrease in autophagy.

Fig. 2.

A) Autophagy activity was measured by the Pho8Δ60 assay in WT, pat1Δ, ski2Δ, sk3Δ pat1Δ ski2Δ, and pat1Δ ski3Δ; strains under growing conditions (+N) and following 3 h of nitrogen starvation (−N). Error bars indicate the standard deviation of 6 independent experiments. ANOVA, **P <0.01 and *** P <0.001. B) Autophagy was measured by GFP-Atg8 processing in WT, pat1Δ, ski3Δ and pat1Δ ski3Δ strains under growing conditions and after 2 h of nitrogen starvation; a representative image is shown. C) Atg1, Atg2-PA, Atg7-PA and Atg9 protein levels were measured by western blot in WT, pat1Δ, ski3Δ and pat1Δ ski3Δ strains under growing conditions and after 1 or 2 h of nitrogen starvation; representative images are shown. D) ATG1, ATG2, ATG7 and ATG9 mRNA levels were determined in WT, pat1Δ, ski3Δ and pat1Δ ski3Δ strains under growing conditions and after 1 h of nitrogen starvation by RT-qPCR. Error bars indicate the standard deviation of 3 independent experiments. ANOVA, ** P<0.01 and *** P <0.001. See also Figure S2.