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. Author manuscript; available in PMC: 2020 Jan 17.
Published in final edited form as: Mol Cell. 2018 Dec 6;73(2):314–324.e4. doi: 10.1016/j.molcel.2018.11.002

Fig. 7. PATL1 levels and phosphorylation state regulate ATG2 and ATG9A mRNA levels and autophagy activity.

Fig. 7.

A) PATL1, ATG2 and ATG9A mRNA levels were measured in Jurkat cells after non-target or PATL1 siRNA treatment. B) Cell lysate from actively growing Jurkat cells was subjected to PATL1 immunoprecipitation followed by mass spectrometry. Identified phosphorylated residues are indicated. C) Jurkat cells were transfected with constructs expressing PATL1 wild-type, AA, EE or empty vector and ATG2 and ATG9A mRNA levels were determined after control and rapamycin treatment. D) Jurkat cells were transfected with constructs expressing PATL1 wild-type, AA or EE and SQSTM1 protein levels were measured through flow cytometry. E) Quantification of the data from (D). Error bars indicate the standard deviation of at least 3 independent experiments. Student’s t-test, ANOVA, *P <0.05, **P <0.01, *** P <0.001.