KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-YFP | Clontech | 632381 |
| Anti-peroxidase (anti-protein A) | Jackson Immunoresearch | 323-005-024 |
| Anti-Pgk1 | Dr. Jeremy Thorner | N/A |
| Anti-Atg1 | In house | N/A |
| Anti-Atg9 | In house | N/A |
| Goat anti Rabbit IgG, HRP | Fisher Scientific | ICN55676 |
| Rabbit anti Mouse IgG, HRP | Jackson Immunoresearch | 315-035-003 |
| Mouse monoclonal IgG2a | Abcam | ab170191 |
| Alexa Fluor 594 goat anti-mouse | Thermo Fisher scientific | A-11005 |
| Anti-PATL1 antibody | Abcam | ab124257 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| 1,10 Phenanthroline | Sigma-Aldrich | 320056 |
| 3-Indole acetic acid | Sigma-Aldrich | I2886 |
| p-Nitrophenyl phosphate | Sigma-Aldrich | N4645 |
| Radiant™ Green 2x qPCR Mix Lo-ROX | Alkali Scientific | QS1020 |
| Complete EDTA-free protease inhibitor cocktail | Roche | 05056489001 |
| IgG Sepharose™ 6 Fast Flow beads | Fisher Scientific | 45-000-173 |
| TURBO DNA-free™ kit | Fisher Scientific | AM1907 |
| RNasin® PLUS RNAse inhibitor | Fisher Scientific | PRN2615 |
| Critical Commercial Assays | ||
| Nucleospin® RNA | Clontech | 740955.250 |
| BCA Protein assay | Fisher Scientific | PI23223, PI23224 |
| Amaxa Cell line Optimization Nucleofector Kit | LONZA | VC0-1001 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Jurkat cells | ATCC | ATCC TIB-152 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: strain background: SEY6210 | See Supp Table 1. | |
| Oligonucleotides | ||
| Primers for RT-qPCR | Hu et al., 2015 | |
| Plasmid Cloning and strain construction primers | See Supp Table 2. | |
| PATL1 siRNA | Dharmacon | E-015591-00-0005 |
| non-target siRNA | Dharmacon | D-001919-10-05 |
| Recombinant DNA | ||
| pMJ160 | This study | |
| pRS-ATG1 (406) | This study | |
| pRS-ATG1 ATG7-3’UTR (406) | This study | |
| pRS-ATG1 ATG17-3’UTR (406) | This study | |
| pRS-ATG1 ATG18-3’UTR (406) | This study | |
| pNHK53 | Morawska and Ulrich. 2013 | |
| pHIS3-AID*-9MYC | Morawska and Ulrich. 2013 | Addgene, 99524 |
| pDZ415 | Hocine et al. 2013 | Addgene, 45162 |
| pCu-MCP-2yeGFP (405) | This study | |
| pDZ274 | Hocine et al. 2013 | Addgene, 45929 |
| pFA6a-VC-His3MX6 | Sung and Huh. 2007 | |
| pCMV-GFP vector | Matsuda and Cepko 2004 | Addgene, 11153 |
| Software and Algorithms | ||
| CFX Manager™ Software | Bio-Rad | |
| Other | ||