|
Antibodies (all
monoclonal rat anti-mouse except where noted) |
| α-CD3 (clone: 17A2) |
Biolegend |
Cat.# 100234 |
| α-CD4 (clone: GK1.5) |
BD Biosciences |
Cat.# 561828 |
| α-CD8a (clone: 53-6.7) |
Biolegend |
Cat.# 100705 |
| α-CD11b (clone: M1/70) |
Biolegend |
Cat.# 101225 |
| α-CD16/32 (Fc receptor block) (clone:
93) |
Biolegend |
Cat.# 101319 |
| α-CD19 (clone: 6D5) |
Biolegend |
Cat.# 115545 |
| α-CD80 (clone: 16-10A1, hamster
anti-mouse) |
BD Biosciences |
Cat.# 563687 |
| α-CD86 (clone: GL-1) |
Biolegend |
Cat.# 105011 |
| α-CD45.1/Ly5a (clone: A20, mouse
anti-mouse) |
Biolegend |
Cat.# 110713 |
| α-CD45.2/Ly5b (clone: 104, mouse
anti-mouse) |
Biolegend |
Cat.# 109805 |
| α-CD169 (clone: MOMA-1) |
Bio-Rad |
Cat.# MCA947F |
| α-F4/80 (clone: T45-2342 |
BD Biosciences |
Cat.# 565411 |
| α-iNOS (clone: CXNFT) |
eBioscience |
Cat.# 12–5920-80 |
| α-Ly6C (clone: HK1.4) |
Biolegend |
Cat.# 128011 |
| α-Ly6G (clone: 1A8) |
Biolegend |
Cat.# 127633 |
| α-NK1.1 (clone: PK136, mouse
anti-mouse) |
Biolegend |
Cat.# 108737 |
| α-TCR Vα2 (clone: B20.1) |
Biolegend |
Cat.# 127807 |
| IgG2a, k isotype control |
Biolegend |
Cat.# 400507 |
| Chemicals, Peptides, and
Recombinant Proteins |
| 1,2-distearoyl-sn-glycero-3-phosphocoline (DSPC) |
Avanti Polar Lipids Inc. |
SKU 850365P |
| 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene
glycol)-2000] (ammonium salt) (DSPE-PEG) |
Avanti Polar Lipids Inc. |
SKU 880128P |
| 3-(N-succinimidyloxyglutaryl)aminopropyl
polyethyleneglycol-carbamyl distearoylphosphatidyl-ethanolamine
(NHS-PEG-DSPE) |
NOF America Corp. |
DSPE-034GS |
| 9-N-(4H-thieno[3,2-c]chromene-2-carbamoyl)-Neu5Acα2–3Galβ1–4GlcNAc-PEG-DSPE
(CD169L) |
This study |
N/A |
| AF555-PEG-DSPE |
Prof. Matthew Macauley |
N/A |
| Cholesterol |
MilliporeSigma |
SKU C8667 |
| Lipopolysaccharide (LPS) (E.
coli 0127:B8) |
MilliporeSigma |
SKU L3129 |
| Chicken ovalbumin (OVA) |
Worthington Biochemical Corp. |
Product Code OAEF |
| O.C.T. medium |
Tissue-Tek (Sakura®) |
Item # 4583 |
| Anti-fade mounting medium |
Vector Laboratories Inc. |
Cat.# H-1000 |
| Cell Trace Violet (CTV) |
Invitrogen |
Cat.# C34557
|
| Critical Commercial
Assays |
| LavaPep protein quantification kit |
Gel Company Inc. |
LP022010 |
| Experimental Models: Cell
Lines |
| THP-1 (wild type) |
ATCC |
TIB-202 |
| THP-1 (CD169 over-expressers) |
Hans Rempel and Lynn Pulliam |
N/A |
| Experimental Models:
Organisms/Strains |
| C57BL/6J mice |
Scripps rodent breeding colony |
N/A |
| CD57BL/6J CD169−/− mice |
Paul Crocker |
N/A |
| C57BL/6-Tg(TcraTcrb)1100Mjb/J,
Ly5a+/− (OT-I, CD8+) |
The Jackson Laboratory (bred to
Ly5a+/+ in house) |
Stock#: 003831 |
| B6.Cg-Tg(TcraTcrb)425Cbn/J, Ly5a+/+
(OT-II, CD4+) |
The Jackson Laboratory (bred to
Ly5a+/− in house) |
Stock#: 004194 |
| Software and
Algorithms |
| FlowJo v. 10 |
BD |
https://www.flowjo.com |
| Prism v. 7 |
Graph Pad Software |
https://www.graphpad.com/scientific-software/prism/ |
| Image Pro Premier v 9.2 |
Media Cybernetics Inc. |
http://www.mediacy.com/imagepro |
| Zen Black & Blue |
Zeiss Inc. |
https://www.zeiss.com/microscopy/int/downloads/zen.html |
| Image J |
NIH |
https://imagej.nih.gov/ij/ |
| Other |
| Membrane supports for liposome extrusion |
Whatman plc./Fisher |
Cat.# NC9759169 |
| Membrane (0.8 μm) for liposome
extrusion |
Whatman plc./Fisher |
Cat.# 09-800-945 |
| Membrane (0.2 μm) for liposome
extrusion |
Whatman plc./Fisher |
Cat.# 05-715-115 |
| Membrane (0.1 μm) for liposome
extrusion |
Whatman plc./Fisher |
Cat.# 09-805-346 |
