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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Neurochem Int. 2018 Sep 1;123:13–21. doi: 10.1016/j.neuint.2018.08.015

Table 1:

Vmax and Km values determined for DA transport in various cell lines.

Cell Lines Vmax
(pmol/min/mg)
Km
(μM)
Reference
rDAT
LLC-PK1 300 - 1925 1.5 - 5.2 (Foster et al., 2012; Foster and Vaughan, 2011; Gu et al., 1994)
COS-7 0.004 - 147 1.5 - 3.5 (Dar et al., 2006, 2005; Lee et al., 1996; Lin et al., 2000; Mazei-Robison and Blakely, 2005)
CHO 348 ± 22 1.7 ± 0.3 (Morón et al., 2003)
mDAT
MDCK - 2.0 ± 0.3 (Wu and Gu, 1999)
rmkDAT
HEK 14.4a 2.9 (Kaufman and Madras, 1993)
hDAT
Oocytes 6 – 66b 7 – 8 (Doolen and Zahniser, 2001; Mayfield et al., 2001; Zhu et al., 1997)
LLC-PK1 4.5 ± 1.2 0.8 ± 0.1 (Zhen and Reith, 2016)
MDCK - 2.4 ± 0.4 (Wu and Gu, 1999)
C17.2c 180 ± 5 5 ± 1 (Grammatopoulos et al., 2010)
CHO 1.0 - 15.7a 0.7 - 2.2 (Cartier et al., 2015; Hamilton et al., 2015; Midde et al., 2015; Slusher et al., 1997)
N27 99 ± 18 2.8 ± 0.4 (Wu et al., 2017)
HEKd 0.2 - 61.2 0.1 - 2.6 (Carvelli et al., 2002; Chen et al., 2003, 2000, 1999; Guptaroy et al., 2011; Jones et al., 2012; Li et al., 2017)
N2a 5.5 - 6.9 0.4 - 4.3 (Mikelman et al., 2017; Zhang et al., 1998)
PC12 6.9 - 12.4a 1.3 - 4.8 (Huang et al., 1999; Melikian and Buckley, 1999; Yuan et al., 2016)
SH-SY5Y 2.1 - 8.9 0.4- 1.4 (Bermingham et al., 2017; Hara et al., 2013; Jiang et al., 2004)
SK-N-MC 45 ± 88 1.1 -1.2 (Pifl et al., 1993; Sweeney et al., 2017)
a

pmol/min/105 cells

b

fmol/sec

c

Mouse multipotent neural progenitor cells

d

Some FLAG tagged