KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-GAPDH clone 6C5 | Millipore | MAB374 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant T. gondii TgPPT1W | (Child et al., 2013) | N/A |
| Recombinant T. gondii TgPPT1S128A | (Child et al., 2013) | N/A |
| Recombinant human HsAPT1WI | (Child et al., 2013; Garland et al., 2018) | N/A |
| Recombinant human HsAPT2WI | (Child et al., 2013; Garland et al., 2018) | N/A |
| Recombinant phospholipase A2 (PLA2) EC 3.1.1.4 | Sigma Aldrich | Cat#P6534 |
| Recombinant trypsin EC 3.4.21.4 | Sigma Aldrich | Cat#T 1426 |
| Recombinant papain EC 3.4.22.2 | Sigma Aldrich | Cat#P4762 |
| Recombinant monoacylglycerol lipase (MGLL) EC 3.1.1.23 | Creative Biomart | Cat#MGLL-2863H |
| Collagenase IV EC 3.4.24.3 | Worthington Biochemical Corp. | Cat#LS004186 |
| Hexadecylfluorophosphonate HDFP | This paper | N/A |
| JCP174 | (Child et al., 2013) | N/A |
| Palmostatin B | Sigma Aldrich | Cat#178501 |
| ML348 | (Adibekian et al.,2010) | N/A |
| ML349 | (Adibekian et al.,2010) | N/A |
| fluorophosphonate-rhodamine (FP-Rho) | (Lentz et al., 2016) | N/A |
| hexadecylsulfonyl fluoride (HDSF) | Santa Cruz Biotech. | Cat# sc-221708 CAS# 86855–26-7 |
| phenylmethylsulfonyl fluoride (PMSF) | Sigma Aldrich | Cat# P7626 CAS#329–98-6 |
| 4-nitropehyl octanoate (4-NPO) | Sigma Aldrich | Cat#21742–1G-F CAS#1956–10-1 |
| Quenched substrate for thioesterases QStE | This paper | N/A |
| Quenched substrate for esterases QSE | This paper | N/A |
| Critical Commercial Assays | ||
| BCA protein assay kit | Pierce | Cat# 23225 |
| Experimental Models: Cell Lines | ||
| Human: ovary adenocarcinoma cell line SKOV3 | (Child et al., 2013; Garland et al., 2018) | ATCC HTB-77 |
| Human: prostate adenocarcinoma cell line PC-3 | (Child et al., 2013; Garland et al., 2018) | ATCC CRL-1435 |
| Human: prostate carcinoma cell line LnCaP | (Child et al., 2013; Garland et al., 2018) | ATCC CRL-1740 |
| Human: ovary adenocarcinoma cell line OVCAR-3 | (Child et al., 2013; Garland et al., 2018) | ATCC HTB-161 |
| Human: breast adenocarcinoma cell line MDA-MB-231 | (Child et al., 2013; Garland et al., 2018) | ATCC HTB-26 |
| Human: breast adenocarcinoma cell line MCF7 | (Child et al., 2013; Garland et al., 2018) | ATCC HTB-22 |
| Experimental Models: Organisms/Strains | ||
| T. gondii strain ΔKu80 | (Child et al., 2013) | N/A |
| T. gondii strain ΔKu80Δppt1 | (Child et al., 2013) | N/A |
| Mouse: C57BL/6 MMTV-PyMT strain B6.FVB- Tg(MMTV-PyVT)634Mul/LellJ) | Jackson Laboratories | 022974 |
| Other | ||
| Syro II automated parallel peptide synthesizer | Biotage | N/A |
| HPLC 1260 Infinity System | Agilent Technologies | N/A |
| Finnigan LTQ mass detector | Thermo Fischer Sci. | N/A |
| HPLC 1100 series | Agilent Technologies | N/A |
| API 150EX single-quadrupole mass spectrometer | Applied Biosystems | N/A |