Figure 4 .

Dispensable role of Stat3 in Wnt-driven intestinal tumorigenesis. A) Schematic illustration of Tam treatment of Apc^Δ716 mice (n = 10) and Apc^∆716 villin-CreER Stat3^flox/flox mice (n = 12) (top). Representative histology sections of small intestinal tumors of Apc^Δ716 mice (left) and Apc^Δ716 Stat3^ΔIEC mice (right) for H&E (top) and immunohistochemical staining for Ki-67 (middle) and phosphorylated Stat3 (P-Stat3) (bottom). Insets show enlarged views. Scale bars, 200 μm. B) Total numbers of polyps in each mouse are shown with dots with mean values (top). Size distribution is shown in bar graph (means ± sd). C) Schematic illustration of Tam treatment of Apc^Δ716 villin-CreER Tgfbr2^flox/flox mice (n = 10) and Apc^Δ716 villin-CreER Tgfbr2^flox/flox Stat3^flox/flox mice (n = 8) (top). Representative histology sections of small intestinal tumor of Apc^Δ716 Tgfbr2^ΔIEC mice (left) and Apc^Δ716 Tgfbr2^ΔIEC Stat3^ΔIEC mice (right) for H&E (top) and immunohistochemical staining for P-Stat3 (bottom). Insets show enlarged views of boxed areas. Scale bars, 400 μm. D) Total numbers of polyps (top) and invasive polyps (bottom) per section are shown with mean numbers.