TABLE 1.
A summary of the mRNA expression of sensory mediators and inflammatory cytokines in the DRG and the skin
| Sensory mediator | Vaseline (copies/μl) | Aldara (copies/μl) | ||
|---|---|---|---|---|
| Vehicle | RTX | Vehicle | RTX | |
| DRG | ||||
| TRPV1 | 1899.9 ± 663.8 | 305.5 ± 126.4### | 1536.2 ± 664.6 | 173.2 ± 51.1### |
| TRPA1 | 182.6 ± 58.1 | 91.9 ± 25.6### | 170.1 ± 56.0 | 71.1 ± 15.2### |
| SP | 8564.8 ± 2057.3 | 1870.7 ± 557.2### | 6366.5 ± 4347.1 | 1771.3 ± 754.7### |
| α-CGRP | 5070.9 ± 988.1 | 2375.8 ± 886.3### | 1579.7 ± 442.2*** | 1364.6 ± 521.2*** |
| TRPM8 | 257.1 ± 139.2 | 231.3 ± 84.5 | 276.6 ± 152.2 | 202.4 ± 69.9 |
| TLR7 | 94.2 ± 62.7 | 118.0 ± 79.7 | 23.4 ± 44.1 | 47.5 ± 67.5 |
| TLR3 | 66.3 ± 36.6 | 145.8 ± 125.5 | 129.9 ± 92.0 | 111.9 ± 86.2 |
| Skin | ||||
| TRPV1 | 11.5 ± 13.8 | 8.1 ± 10.6 | 13.4 ± 12.8 | 38.1 ± 30.0 |
| TRPA1 | 1.2 ± 0.9 | 1.2 ± 1.5 | 0.7 ± 0.5 | 1.3 ± 1.2 |
| SP | 26.2 ± 31.7 | 26.6 ± 21.1 | 4.9 ± 7.1 | 10.7 ± 9.3 |
| α-CGRP | 48.7 ± 15.8 | 70.5 ± 45.2 | 30.4 ± 9.5 | 40.1 ± 18.8 |
| TRPM8 | 6.1 ± 8.5 | 5.8 ± 4.8 | 2.5 ± 5.8 | 3.7 ± 4.4 |
| IL-1β | 359.2 ± 146.3 | 431.4 ± 340.2 | 1266.8 ± 619.2 | 1758 ± 812.4 |
| IL-8 | 119.4 ± 26.5 | 89.11 ± 22.9 | 154.7 ± 34.1 | 69.3 ± 18.0### |
| IL-6 | 359.3 ± 73.5 | 392.2 ± 109.0 | 453.7 ± 159.8 | 401.7 ± 100.3 |
| TNF-α | 145.0 ± 37.1 | 162.2 ± 71.6 | 123.7 ± 55.4 | 149.3 ± 67.9 |
| CCL5 | 549.9 ± 289.9 | 332.3 ± 224.0 | 515.8 ± 378.7 | 62.9 ± 48.1**,# |
| IL-17A | Too few for analysis | Too few for analysis | Too few for analysis | Too few for analysis |
| IL-23 | Too few for analysis | Too few for analysis | Too few for analysis | Too few for analysis |
Data represent means ± sd and were analyzed by 2-way ANOVA with Bonferroni’s post hoc test. n = 5–7 animals/group. **P < 0.01, ***P < 0.001 Vaseline vs. Aldara, #P < 0.05, ###P < 0.001 vehicle vs. RTX.