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. 2018 Oct 11;33(2):2759–2769. doi: 10.1096/fj.201801163R

Figure 4 .

Figure 4

LIN28B regulates trophoblast proliferation, differentiation, inflammation, and C19MC miRNA expression. A) Representative immunoblot, densitometric quantification, and qPCR analysis of LIN28B levels 72 h after control or LIN28B shRNA-mediated knockdown in JEG3 cells. B, C) qPCR analysis of indicated gene (B) and miRNA (C), normalized to GAPDH and U18, respectively, after transient transfection of JEG3 cells with control or LIN28B shRNA. D) Representative immunoblot, densitometric quantification, and qPCR analysis of LIN28B levels 72 h after transient overexpression of GFP (control) or LIN28B encoding plasmids in HTR8/SVneo cells. E) qPCR analysis of indicated genes 72 h after transient overexpression of GFP or LIN28B in HTR8/SVneo cells. F) Cell proliferation analysis 72 h after transient knockdown of LIN28B in JEG3 cells or transient overexpression of LIN28B in HTR8/SVneo cells. Results represent means ± sem. *P < 0.05 shLIN28B vs. scrambled (A–C) or LIN28B vs. control (D, E).