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. 2018 Sep 11;33(2):1946–1962. doi: 10.1096/fj.201800683RR

Figure 4 .

Figure 4

Inactivation of PERK causes a slow-to-fast fiber-type transition in adult mice. A) Muscle sections prepared from soleus muscle of control (Ctrl) and smPERK-KO mice were subjected to triple immunostaining against MyHCI, IIa, and IIb proteins. Representative photomicrographs of triple-stained sections of soleus muscle. Scale bar, 50 μm. B, C) Quantification of percentage of each fiber type (B) and mean myofiber CSA of type I and IIa fibers (C) in soleus muscle of Ctrl and smPERK-KO mice (n = 6/group). D) Muscle sections prepared from TA muscle of Ctrl and smPERK-KO mice were subjected to triple immunostaining against MyHCI, IIa, and IIb proteins. Representative photomicrographs of triple-stained TA muscle sections are presented here. Scale bars, 50 μm. E, F) Quantification of percentage of each fiber type (E), and mean myofiber CSA of type IIa, and IIb (F) fibers in TA muscle of Ctrl and smPERK-KO mice (n = 3–5/group). Error bars ± sd. *P < 0.05 vs. corresponding littermate Ctrl (unpaired, 2-tailed Student’s t test).