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. 2018 Sep 11;33(2):1946–1962. doi: 10.1096/fj.201800683RR

Figure 6 .

Figure 6

Ablation of PERK activates calpains in skeletal muscle. A) Representative photograph and quantification of bands of μ- and m-calpain in a casein zymogram performed on GA muscle extracts from control (Ctrl) and smPERK-KO mice (n = 4/group). B) Enzymatic activity of calpains in GA muscle of Ctrl and smPERK-KO mice (n = 4 per group). C) Relative mRNA levels of calpain 1, calpain 2, and calpastatin in GA muscle of Ctrl and smPERK-KO mice (n = 4–5/group). D) Differentiated myotubes were incubated with vehicle alone (DMSO) or 2 µM GSK2606414 for 48 h, and protein extracts were analyzed for calpain activity (n = 8/group). Error bars ± sd. *P < 0.05, **P < 0.01 vs. corresponding littermate Ctrl mice (unpaired, 2-tailed Student’s t test).