Figure 3 .

AQP2 expression and localization in Epac1^−/− and Epac2^−/− mice. A) Representative Western blots from whole kidney lysates of Epac WT, Epac1^−/−, and Epac2^−/− mice probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1^−/−, and Epac2^−/− mice from Western blots similar to that shown in A. Intensity values were normalized to total signal of respective lines in Ponceau red staining (Supplemental Fig. S1). *P < 0.05 vs. Epac WT. Number of mice in each group is indicated on each bar. C) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse-cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1^−/−, and Epac2^−/− mice. Nuclear DAPI staining is shown in pseudocolor blue.