Western blot of Ca2+ pump proteins in SR from control and MO 0.9 gestation fetal heart LVs. SDS-PAGE gel image of pRyR2(Ser2808), pRyR2(Ser2814), RyR(pan), FKBP12.6, Serca-2, pPLN(Ser16), and PLN(pan) (A); pRyR2(Ser2808) expression (B); pRyR2(Ser2814) expression (C); RyR (pan) expression (D); pRyR2(Ser2808)-to-RyR(pan) ratio (E); pRyR2(Ser2814)-to-RyR(pan) ratio (F); FKBP12.6 expression (G); Serca2 expression (H); pPLN(Ser16) expression (I); PLN(pan) expression (J); and pPLN-to-PLN(pan) ratio (K). Means ± sem.(B–D and G–J,
n = 6 control fetal hearts and n = 7 MO fetal hearts, normalized to β-actin expression; E, F, and K normalized to relative pan protein expression). *P < 0.05 vs. control.