Figure 5 .

Isoform-specific regulation of MCPH1 by APC/C–CDH1. A) HeLa Tet-On cells were cotransfected with GFP–MCPH1-L and either FLAG empty vector or FLAG–CDH1. GFP–MCPH1-L expression was induced by the addition of tetracycline 10 ng/ml. After 24 h, cells were lysed and samples analyzed by using Western blotting. B) HeLa Tet-On cells were cotransfected with GFP–MCPH1-L or GFP–MCPH1-S, along with either FLAG empty vector or FLAG–CDC20 or FLAG–CDH1. GFP–MCPH1 expression was induced and samples processed as in A. C, D) HeLa Tet-ON cells were cotransfected with the indicated plasmids and processed as in A.