TABLE 1.
Phosphorylation sites identified in GFP–MCPH1-L from nocodazole-treated cells
| Peptide | Amino acid | Previously published |
|---|---|---|
| TFTTQLVDMGAK | T27 | No |
| KLEGSINDIK | S254 | Yes |
| ANNIHSSPSFTHLDK | S276 | Yes |
| ANNIHSSPSFTHLDK | S277 | Yes |
| ANNIHSSPSFTHLDK | S279 | Yes |
| YRLSPTLSSTK | S333 | Yes |
| YSENLPPESQLPSSPAQLSCR | S437 | Yes |
| YSENLPPESQLPSSPAQLSCR | S438 | Yes |
| GTLFADQPVMFVSPASSPPVAK | T754 | No |
| GTLFADQPVMFVSPASSPPVAK | S769 | No |
| QASIVIGPYSGK | S801 | No |
HEK293 cells were transfected with GFP–MCPH1-L for 24 h before nocodazole treatment (100 ng/ml, 20 h). GFP–MCPH1 was purified from cells after mitotic shake-off and digested with trypsin/pronase. Peptides were analyzed by mass spectrometry using a Sciex TripleTOF 5600. The phosphorylated peptides are listed, with the site of phosphorylation underlined.