. 2019 Jan 18;2:23. doi: 10.1038/s42003-018-0275-4

Table 2.

shRNA sequences for RNAi experiments

siRNA	Sequence
siGFP	Sense: gatccccGGAGTTGTCCCAATTCTTGttcaagagaCAAGAATTGGGACAACTCCtttttggaa
siGFP	Anti-sense: agcttttccaaaaaGGAGTTGTCCCAATTCTTGtctcttgaaCAAGAATTGGGACAACTCCggg
Mouse siMEP50-m1	Sense: gatccccGCAAGGACTTGCATTGTTAttcaagagaTAACAATGCAAGTCCTTGCttttta
Mouse siMEP50-m1	Anti-sense: agcttaaaaaGCAAGGACTTGCATTGTTAtctcttgaaTAACAATGCAAGTCCTTGCggg
Mouse siMEP50-m2	Sense: gatccccGCATGCATGGAACGTCAATttcaagagaATTGACGTTCCATGCATGCttttta
Mouse siMEP50-m2	Anti-sense: agcttaaaaaGCATGCATGGAACGTCAATtctcttgaaATTGACGTTCCATGCATGCggg
Mouse siPRMT5-m1	Sense: gatccccGACTCTAGTATCAAGGAAttcaagagaTTCCTTGATACTAGAGTCCttttta
Mouse siPRMT5-m1	Anti-sense: agcttaaaaaGACTCTAGTATCAAGGAtctcttgaaTTCCTTGATACTAGAGTCCggg
Mouse siPRMT5-m2	Sense: gatccccGGAAACAAATAAAGTGAAGttcaagagaCTTCACTTTATTTGTTTCCttttta
Mouse siPRMT5-m2	Anti-sense: agcttaaaaaGGAAACAAATAAAGTGAAGtctcttgaaCTTCACTTTATTTGTTTCCggg
Human siControl	Sense: gatccccATCTCGCTTGGGCGAGAGTAAGttcaagagaCTTACTCTCGCCCAAGCGAGATttttta
Human siControl	Anti-sense: agcttaaaaaATCTCGCTTGGGCGAGAGTAAGtctcttgaaCTTACTCTCGCCCAAGCGAGATggg
Human siMEP50	Sense: gatccccGGACTCTGTGTTTCTTTCAttcaagagaTGAAAGAAACACAGAGTCCttttta
Human siMEP50	Anti-sense: agcttaaaaaGGACTCTGTGTTTCTTTCAtctcttgaaTGAAAGAAACACAGAGTCCggg
Human siPRMT5	Sense: gatccccGGAATCTCAGACATATGAAGTttcaagagaACTTCATATGTCTGAGATTCCttttta
Human siPRMT5	Anti-sense: agcttaaaaaGGAATCTCAGACATATGAAGTtctcttgaaACTTCATATGTCTGAGATTCCggg
Human siGLI1	Sense: gatccccGCAGTAAAGCCTTCAGCAAttcaagagaTTGCTGAAGGCTTTACTGCttttta
Human siGLI1	Anti-sense: agcttaaaaaGCAGTAAAGCCTTCAGCAAtctcttgaaTTGCTGAAGGCTTTACTGCggg

siGFP and non-targeting control siRNA (siControl) were used as controls in RNAi experiments