Fig. 2.

Linked-reads data validation for dmV, dmVI, and dmVII in the relapse sample of SJHGG019. a Validation of dmV. The heat maps above dmV represent barcode sharing of the linked-reads between two immediately joined segments, and the heat maps below dmV represent barcode sharing of the linked-reads between two segments spaced by another segment. The red arrows represent the SVs validated by barcode sharing of linked-reads with strong evidence. The gray arrows represent the SV validated by barcode sharing of linked-reads with less strong evidence due to the long distance in between. The scale of the segments’ lengths is slightly adjusted to accommodate all the heat maps. b Invalidation of dmVI and dmVII. The heat maps represent barcode sharing of the linked-reads between two immediately joined segments. While seg27 is indeed linked with 5′ of seg9 in dmVI, it stops at 3′ of seg9 and does not extend to seg10 as predicted in the structure. Similarly, while seg19 is indeed linked with 3′ of seg9 in dmVII, it stops at 5′ of seg9 and does not extend to seg8 as predicted in the structure