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. 2019 Jan 18;9:227. doi: 10.1038/s41598-018-36698-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Normalized output fraction of osteogenically primed SVF and A375 controlled-ratio sorts using FACS, manufacturer’s protocol MACS, and optimized protocol MACS. Defined mixtures of osteogenically primed SVF and A375 cells were labeled with anti-ALPL-APC antibodies (and anti-APC microbeads for MACS) prior to cell sorting. After separation, ALPL+/retained and ALPL−/flow-through fractions were counted and normalized output fractions computed. For ALPL+:ALPL− cell ratios >1:3, manufacturer’s protocol MACS sorts were not able to accurately separate ALPL+ and ALPL− cells. Error bars indicate standard deviation.