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. 2019 Jan 18;9:227. doi: 10.1038/s41598-018-36698-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell yield and fraction analyses for MACS optimization trials of varying antibody and microbead concentrations. MACS cell yields of retained and flow-through fractions for pure ALPL+ optimization sorts when (a) microbead dilution was varied with a fixed (1:11) antibody dilution and (b) antibody dilution was varied with a fixed (1:5) microbead dilution. (c) Distributions of ALPL+ cells within retained and flow-through fractions of antibody-varied trials displayed in (b) were assessed via flow cytometry and confirmed a high false-negative rate in flow-through fractions. (APC fluorescence corresponds to APC dye on anti-ALPL antibody).