Fig. 2.

E47 knockout mice are protected from GC-induced side effects. a i.p. glucose tolerance test (GTT) and b i.p. pyruvate tolerance test (PTT) of E47−/− and WT mice on Dex. Data were analyzed by ANOVA and Bonferroni’s multiple comparison test and are shown as mean ± SEM. Asterisks indicate significance, *P < 0.05, n = 10 per genotype (GTT); n = 7 (WT) & 9 (E47−/−) (PTT). c qRT-PCR of gluconeogenic genes in Dex-treated livers, normalized to U36b4; data are mean ± SEM *P < 0.05, Student’s t test. n = 6 (WT) & 5–6 (E47−/−). d Liver sections stained with Oil Red O and hematoxylin and eosin (H&E) after Cort or vehicle treatment. ×20 magnification; black scale bar: 100 μm, representative image from n = 3. e Liver and plasma triglycerides on Cort. Data are mean ± SEM, n = 6 (WT) and 4–6 (E47−/−). f Volcano plot and GO analysis of deregulated genes (blue = downregulated; red = upregulated, FC 1.3, P < 0.05) in livers of E47−/− mice on Dex, n = 2 (WT) & 3 (E47−/−). For GO analysis a base mean cutoff > 200 was used. g qRT-PCR of metabolic genes on Dex, normalized to U36B4. Data are mean ± SEM, *P < 0.05, Student’s t test, n = 5–7 (WT) & 4–7 (E47−/−). h Volcano plot and GO analysis of deregulated genes (blue = downregulated; red = upregulated, FC 1.3, P < 0.05) in livers of E47−/− mice on Cort, n = 4 per genotype. For GO analysis a base mean cutoff > 200 was used. i qRT-PCR of differentially expressed genes on Cort, normalized to U36b4; data are mean ± SEM *P < 0.05, Student’s t test. n = 4 per genotype