Fig. 4.
E47 is required for efficient chromatin binding of GR, FoxO1 and Mediator. a ChIP-MS for GR in Dex-injected control livers. Pale blue: chromatin remodelers (GO Biol. Process); green: NR coregulators; dark blue: sequence specific DNA binding transcription factor activity (GO Mol. Function); dark grey: significant outliers derived by Fisher’s exact test (FDR < 0.05, s0 = 1).; n = 3 biological replicates. b ChIP-MS for GR in Dex-injected E47ΔLKO livers. Pale blue: chromatin remodelers (GO Biol. Process); green: NR coregulators; dark blue: sequence specific DNA binding transcription factor activity (GO Mol. Function); dark grey: significant outliers derived by Fisher’s exact test (FDR < 0.05, s0 = 1).; n = 3 biological replicates. c ChIP-qPCR in Dex-treated E47−/− and wild-type livers shows binding of GR, Med1 and FoxO1 at metabolic promoters and enhancers. The data are shown as fold enrichment over IgG and normalized to a negative control region. Data are mean ± SEM, *P < 0.05, **P < 0.01 Student’s t test, n = 6, ns not significant