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. 2019 Jan 18;5:55. doi: 10.1038/s41420-018-0136-4

Fig. 6. Chondrocyte phenotypic stability assay, tumourigenicity assay and karyotype analysis for piCLCs.

Fig. 6

a Cell morphologies of passage 5 (P5) and passage 21 (P21) piCLCs. b Toluidine blue staining and alcian blue staining for early P5 and late P21 piCLCs. c, d Soft-agar colony formation assay for CNE2 cells (c) and piCLCs (d). c Soft-agar colony formation assay for CNE2 cells. CNE2 cells, a nasopharyngeal carcinoma cell line, were used as a positive control. d Soft-agar colony formation assay for piCLCs (103 cells/well, 5 × 103 cells/well and 104 cells/well). e Karyotype analysis for P5 and P21 piCLCs