Figure 7.

Seven days of CS exposure does not increase ER Stress proteins in AM from RAGE null mice. Western blotting was performed on AM proteins using antibodies targeting protein involved in the ER stress response including protein kinase R-like endoplasmic reticulum kinase (PERK), inositol requiring enzyme 1α (IRE1α) and protein disulfide isomerase family A member 3 (PDIA3). Cell lysates were pooled from 3–4 male mice per group. Immunoblots show the basal level of each ER stress protein was higher in control RAGE null compared to WT mice. However, CS exposure increased the expression of all three proteins in only WT mice. Full length gels are shown in Supplementary Fig. 7.