Fig. 6.

Agrp neuron activity boosts fat gain in obesogenic conditions. a Physiological hyperphagia after acute switch to high-fat diet (45% kcal from fat) in mice. b Pair feeding studies: high-fat diet (0, 25 or 50% more calories compared to ad libitum normal chow food intake) was provided after injection of control and Agrp^Trpv1 mice with capsaicin. c Top, Mice were fed the same number of calories as they ate during baseline food intake; Middle, delta body weight; Bottom, metabolic efficiency. d Similar to c but in mice fed 25% more calories when switched to a high-fat diet. e Similar to c and d, but in mice fed 50% more calories. f Food intake measured during 5 days after switching mice to a high-fat diet (45% kcal from fat); in pink, period of dietary switch. g Control and Agrp^Trpv1 mice were switched to a high-fat diet (shaded pink) after baseline food intake measurements; capsaicin was injected every day before dark cycle. h Food intake. i Delta changes in body weight relative to the day of diet switch. j Metabolic efficiency and k gain in fat mass during the period in which mice were fed high-fat diet. Unpaired t-test was used in c, d, e, j and k. Two-way analysis of variance (ANOVA with time as a repeated measure was used in i. Number of mice is displayed in the figures. Bars and symbols indicate mean ± SEM. Boxes indicate median ± 25/75 quartiles ± min/max values. Statistically significant P values are provided in the figures