Fig. 3.

Outflow perfusate concentration-time profiles. Concentration (nM) vs. time (min) profiles of (A) TVP, (B) DM-4103, and (C) DM-4107 in the outflow perfusate of isolated perfused livers (IPLs) from WT and PCK rats. Statistically significant differences in concentrations between WT and PCK IPLs were assessed at t = 30 and 60 min. Perfusions with tolvaptan (∼7 μM) and subsequently with blank buffer (washout) for 30 min each are represented by the black and gray bars, respectively. Data are presented as mean ± S.D. (n = 6 for WT; n = 5 for PCK). *P < 0.05, PCK vs. WT.