Fig. 2.

Mcm10 stimulates CMG helicase, followed by reannealing of the unwound products. (A, Left) Schematic of the assay. (A, Right) Unwinding time courses of 30 nM CMG with and without mixing with 60 nM Mcm10 with an oligo trap (shown in blue), analyzed by native PAGE. (B) Schematic of the assays for C and D. (C) Unwinding time courses of 30 nM CMG with no oligo trap with and without mixing with 60 nM Mcm10. The substrate was partially shifted by Mcm10 and/or CMG, resulting in a doublet band. (D) Unwinding time courses of 50 nM CMG or 50 nM preformed CMGM complex with no oligo trap.