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Mcm10 promotes robust strand annealing. (A) Schematic of the assay. The lagging strand oligo of the forked DNA was 5′ end-labeled with 32P. (B) Time course of strand annealing using 60 nM Mcm10 and analyzed by native PAGE. (C) Quantitation of annealing vs. time in the presence or absence of Mcm10. Each data point is the average of three separate reactions. Error bars represent 1 SD.
