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Mcm10 can anneal RPA-coated single strands. (A) Native PAGE EMSA of Mcm10 binding the radiolabeled 5′ flap lagging-strand oligonucleotides used in annealing reactions. (B) Annealing assays with and without 60 nM Mcm10, with increasing concentrations of RPA precoating the ssDNA. Gel loading buffer contains SDS to dissociate protein from DNA. (C) Quantitation of reannealing reactions. Values are the average of three experiments. Error bars represent 1 SD.
