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. 2018 Dec 31;116(3):798–803. doi: 10.1073/pnas.1819107116

Fig. 6.

Fig. 6.

Mcm10 inhibits SMARCAL1 catalyzed fork regression. Fork regression assays of SMARCAL1 with increasing Mcm10 added. (A) 2 nM SMARCAL1 on a lagging single-strand gap substrate at 2 min of reaction. (B) 5 nM SMARCAL on a leading single-strand gap substrate at 5 min of reaction. The two arms of the forked DNA are complementary, and substantial spontaneous regression is prevented by two mismatches at the fork junction, indicated by a kink in the duplex. Quantitation is the average of triplicate experiments. Error bars represent 1 SD.