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. 2018 Dec 31;116(3):934–943. doi: 10.1073/pnas.1816822116

Fig. 3.

Fig. 3.

Evidence for a novel genetic-code change in Balanophora plastomes. (A) Structure of the clpP and rpl2 genes. The approximate location of six codons diagnostic of a code change are given. Exons are represented by boxes and introns by interrupted lines. (B) Partial alignments of the inferred amino acid sequences of CLPP and RPL2. The histograms indicate the percentage of the 18 sequences in the alignment that share the most common amino acid at each position. (C) Pairwise dN/dS ratios from sliding-window analysis (window size = 90 bp; step size = 30 bp) of the Balanophora clpP and rpl2 genes (SI Appendix, Fig. S9 shows the same analysis of three other Balanophora genes). Arrows mark internal TAG codons present in one or both Balanophora plastomes and inferred to encode W; note that, at five of these six positions, most or all non-Balanophora land plants contain TGG (W in the standard genetic code). The positions of these TAG codons in the complete alignments are shown in SI Appendix, Fig. S4.