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. 2019 Jan 4;16(1):122. doi: 10.3390/ijerph16010122

Figure 2.

Figure 2

Life-long exposure to CPF regulates the expression of several mRNAs included in the ECGS and genes evidenced by the network analyses. mRNAs were prepared from thyroid of females exposed to 1 mg/kg/day and 10 mg/kg/day of CPF from the conception and life-long. They were sacrificed at 12 months and RNA prepared from thyroid. Level ECGS mRNAs, determined by real time polymerase chain reaction in control (not exposed) and treated animals. Data are reported as fold change values calculated as ratio between average relative gene expression in treated and control mice. Data are reported as the average and standard deviation of Gapdh-normalized mRNA levels of 5 animals. Mean and standard deviation is reported (Dmbt1 p-value = 0.02, p = 0.015; Chd9 p = 0.04; Ddit4 p = 0.025, p = 0.023; Cpeb2 p = 0.043; Znf703 p = 0.026, p = 0.035; Zfp36l2 p = 0.046).