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. 2019 Jan 4;16(1):122. doi: 10.3390/ijerph16010122

Figure 3.

Figure 3

Chronic expression of constitutively activated RAS in immortalized thyrocytes induces several mRNAs included in the ECGS and genes evidenced by the network analyses. RNA was prepared from FRTL-5 cells transformed by mutated Ras (Ha-RasV12), expressing a constitutive Ha-RasV12 oncogene (FRTL-5-RasV12). (A) Level ECGS mRNAs, determined by real time polymerase chain reaction in control (FRTL-5) and transformed cells. (B) p65 and IκBα mRNAs were determined by RT-qPCR in the same samples. Western blotting (C) and densitometry analysis (D) of DMBT1, ZFP36L2 and DDIT4 protein in FRTL-5 wild type and FRTL-5-RasV12 cells. β-Tubulin was used as loading control. Data are reported as fold change values calculated as ratio between average relative gene expression in treated and control cells. Data are reported as the average and standard deviation of Abl-normalized mRNA levels. Results are expressed as the mean ± standard deviation of three independent experiments. (Dmbt1 p-value = 0.0001; Chd9 p = 0.03; Ddit4 p = 0.035; Cpeb2 p = 0.003; Znf703 p = 0.034; Zfp36l2 p = 0.0054).